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Further Molecular Characterization and Effect on Host Photosynthetic Pigments and Carbohydrate Pools of an Egyptian Isolate of TBSV
Faiza A. Fattouh, Awatif S. Ali and Radwa M. Fathy
The Egyptian isolate of Tomato bushy stunt virus, the TBSV Egh was further characterized with respect to its coat protein gene (P42CP), movement protein gene (P22MP) and replication protein gene (P33RP). All 3 genes were amplified by PCR from TBSV Egh purified virus. Both P42CP and P22MP were further cloned, partially sequenced and deposited into the gene bank under accession numbers HM439101 NCBI and JX418297 respectively. Phylogenetic analysis illustrates evolutionary relationship between TBSV Egh P22 and P42 partial sequence indicated that this isolate may represent a new different strain of TBSV. Loss in chlorophyll content of TBSV Egh infected Lycopersicon esculentum and Cucurbita pepo hosts are reported. Variable significant differences in glucose, sucrose and polysaccharides have been observed