海岸带管理杂志

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Phylogenetic Diversity of Secondary Metabolite Producing-Bacteria Associated with Sponges from Bandengan Waters, Jepara

Ocky Karna Radjasa and Agus Sabdono

A total of 13 bacterial isolates associated with sponges collected from Bandengan water,
Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against
pathogenic bacteria Vibrio parahaemolyticus, Aeromonas hydrophila and Staphylococcus aureus.
Active bacterial isolates were rapidly grouped by using rep-PCR and a dendrogram was
constructed. Six isolates were further selected based on the constructed dendrogram for subsequent
DNA sequencings, resulted in the richness of secondary metabolite-producing bacteria associated with
sponges from Bandengan having closest similarity to Pseudoalteromonas, Brachybacterium, Vibrio,
alpha proteobacterium and uncultured bacterium clones.