药物分析学报

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Simultaneous Determination of Eprosartan Mesylate and Hydrochlorthiazide in Pharmaceutical Dosage form by Reverse Phase High Performance Liquid Chromatography

Devika G S, M Sudhakar and J Venkateshwara Rao

A simple, rapid, sensitive and accurate reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of Eprosartan mesylate and hydrochlorthiazide in combination.Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (150 mm× 4.6 mm id, 5μm particle size). The mobile phase comprising of acetonitrile: methanol: 0.01M KH 2 PO 4 buffer (40:40:10) was delivered at a flow rate of 1.0mL/min. The pH of the mobile phase is adjusted to 4 with ortho phosphoric acid .Detection was performed at 270nm.The total run time is 5 min and the retention time of Eprosartan mesylate is 3.56 min and hydrochlorthiazide is 4.62 min respectively.The described method is linear for the assay of Eprosartan mesylate and hydrochlorthiazide over a concentration range of 216-576μ g/mL and 9-24μg/mL respectively. Results of the analysis have been validated and by recovery studies.The excipients present in the formulations do not interfere with the assay procedure.The developed method was successfully applied to determine Eprosartan mesylate and hydrochlorthiazide in pharamaceutical formulations.