临床与实验药理学杂志

抽象的

The determination of metoclopramide and ondansetron in urine samples by Using Electro membrane extraction combined with capillary electrophoresis

Ehsan Sadeghi

Electro membrane extraction (EME) is a sample preparation technique in pharmaceutical, chemical, clinical and environmental analysis. This technique uses electromigration across artificial liquid membranes for selective extraction of analytes and sample enrichment from complex matrices. This method has many advantages such as simplicity, rapid, low-cost, low LOD, high preconcentration factor and high recovery.           In the present work, simultaneous preconcentration and determination of two basic drugs namely metoclopramide (MCP) and ondansetron (OSN) were studied using EME as a suitable extraction method, followed with capillary electrophoresis (CE) using ultraviolet (UV) detection as separation technique. The drugs were extracted from 4 ml sample solutions, through a supported liquid membrane (SLM) consisting 2-nitrophenyloctylether (NPOE) impregnated in the walls of a polypropylene hollow fiber, and into a 20 μL acidic aqueous acceptor solution resent inside the lumen of the hollow fiber with a potential difference applied over the SLM. The variables of interest, such as chemical composition of the organic liquid membrane, stirring speed, extraction time and voltage, pH of donor and acceptor phases and salt effect in the EME process were investigated and optimized. Under optimal conditions NPOE as SLM, stirring rate of 1000 rpm, 200 V potential differences, 20 min as the extraction time, acceptor phase HCl (pH 1.0) and donor phase HCl (pH 1.5).