微生物与生化技术杂志

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Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

Einas Hamed El-S, Magda Mahmoud Ibrahim El-A and Mervat Mounir S

Lectins of five cultivars of Phaseolus vulgaris seeds were isolated using ammonium sulfate precipitation followed by dialysis and their molecular characterization were determined using SDS-PAGE. The suggested regions of all isolated lectins ranged between 31 to 34 kDa. The isolated lectins demonstrated remarkable hemagglutination activity to all human blood groups (A, B, AB and O). The antimicrobial activity was studied using agar-well diffusion method and minimum inhibitory concentration (MIC) was determined. The results showed that the lectins of all tested Phaseolus vulgaris seeds had a potent antibacterial activity against all bacterial strains studied (Staphylococcus aureus ATCC 6538, and Streptococcus mutants ATCC 25175, Pseudomonas aeruginosa ATCC 10145 and Klebsiella pneumonia) with the exception of Escherichia coli 0157: HZ ATCC 51659 that was not affected. All tested lectins showed antifungal activity against Candida albicans. As far as we are aware, our work is the first approach that showed photographs of scanning electron microscope (SEM) before and after treatment of 90% saturated fraction of the lectins extracted from the Egyptian Shalatine cv. seeds against Staphylococcus aureus and Pseudomonas aeruginosa, where a potential agglutination of bacterial cells was shown.