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Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form
Hemendra Kumar Sharma, Nilesh Jain and Surendra Kumar Jain
A new, simple, accurate, precise and reproducible UV spectrophotometric method is being developed for the simultaneous estimation of amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide in tablet dosage form. The stock solutions were prepared in methanol. The λ max for amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide were 238.5nm, 256.5nm and 271.5nm respectively. The amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide obeyed Beer’s law in concentration range of 5-25μg/ml, 6-30μg/ml and 5-25μg/ ml respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.