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Evaluation of Antimicrobial Activity of Water Infusion Plant-Mediated Silver Nanoparticles

Omar El-Shahaby, Mustafa El-Zayat, Ehab Salih, Ibrahim M El-Sherbiny and Fikry M Reicha

Phytochemical analysis revealed that the infusion of Rumex dentatus is rich in flavonoids, phenolics, reducing sugars and total sugars. The plant infusion found to possess a significant antioxidant activity. The aqueous extract of Rumex dentatus has been used as a green reducing and stabilizing agent in addition to its desirable biological activity. The biosynthesis of reduced silver nanoparticles was very fast, and silver nanoparticles were synthesized by exposure of a mixture of different ratios of 4 mM AgNO3 and Rumex dentatus aqueous extract to UV-irradiation at room temperature. Ultraviolet-visible absorption spectroscopy, transmission electron microscopy and X-ray diffraction confirmed reduction of Ag+ ions and formation of silver nanoparticles. Varied morphology of the bioreduced silver
nanoparticles was recorded. On contrast to the main plant infusion, the resulting silver nanoparticles were found to possess wide spectrum of antimicrobial activity increasing proportionally with the increase of the concentration of the silver nanoparticles against wide variety of bacterial and fungal pathogenic strains.