微生物与生化技术杂志

抽象的

New Probiotic Culture of Lactococcus lactis ssp. lactis: Effective Opportunities and Prospects

Murat Zh Nuryshev, Lidia G Stoyanova and Alexander I Netrusov

We have isolated several new strains of lactococci from raw milk of Buryatia region of Russia near Lake Baikal with wide variety of climatic and ecological niches. Physiological and biochemical features of new strains were studied and compared to the nisin-producing strain Lactococcus lactis ssp. lactis MSU. According to morphological, cultural, physiological, biochemical properties and gene sequence of 16S rRNA a novel most effective strain 194 was identified as Lactococcus lactis ssp. lactis (GenBank database DQ 255954), which has status “GRAS” (absolutely harmless for human health and animals). The strain 194 had inhibitory activity against Gram-positive, Gram-negative pathogenic bacteria and also on fungi of genera Aspergillus, Fusarium and Candida. This is unique biological property for natural strains of Lactococcus lactis specie. We also studied the probiotic properties of strain as resistance to HCl and bile acids, sensitivity to antibiotics and show the therapeutic effect of strain as a food additive on model mice CBRB-Rb (8,17) 1Iem chronic dermatitis.