生物等效性和生物利用度杂志

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Single and Multiple Pharmacokinetics of Enrofloxacin and Ciprofloxacin in Pigs

Claudio Araneda, Paulina Villar, Carolina Cuadros, Mauricio del Valle, Pia Nunes and Margarita Santelices

Pharmacokinetics of Enrofloxacin (ENR) and Ciprofloxacin (CPX) in pig plasma was studied by HPLC-FL analysis using two injectable solutions, Enromic ® 20% (single dose of 7.5 mg ENR Kg -1 body weight) and Enromic ® 10% (multiple dose of 2.5 mg ENR Kg -1 body weight/day during 3 consecutive days). For the method validation, standard calibration curves were prepared between 0.025 and 0.5 μg mL -1 , with r 2 >0.9998 for both analytes. Quantification limits were 0.0282 and 0.0289 μg mL -1 for ENR y CPX, respectively; recovery percentages vary between 90.09% and 104.84% for ENR, and 63.01% and 89.01% for CPX, and precision obtained for measurements made on different days, expressed as %RSD, varied between 2.70% and 15.26% for ENR, and between 6.58% and 13.31% for CPX. Pharmacokinetic parameters gave values for Enromic ® 20% of 1.139 ± 0.320 μg mL -1 (C max ), 3.500 ± 1.581 h (T max ) and 17.821 ± 3.020 μg mL -1 h (AUC 0→∞ ) DU for ENR and 0.047 ± 0.010 μg mL -1 (C max ), 9.200 ± 1.932 h (T max ) and 1.027 ± 0.138 μg mL -1 h (AUC 0→∞ ) for CPX . For the product Enromic ® 10% (multiple doses), values were 0.428 ± 0.119 μg mL -1 (C max ), 5.000 ± 0 h (T max ) and 4.616 ± 1.138 μg mL -1 h (AUC 0→∞ ) for ENR and for CPX were 0.023 ± 0.006 μg mL -1 (C max ), 6.000 ± 2.108 h (T max ) and 0.424 ± 0.129 μg mL -1 h (AUC 0→∞ ) . We conclude that the most analyzed parameters are similar for both products, regardless of their different administration regime.