水产养殖研究与发展杂志

抽象的

Stock Structure Delineation of the African Catfish (Clarius gariepinus) in Selected Populations in Kenya Using Mitochondrial DNA (Dloop) Variability

Cynthia Nyunja, Joyce Maina, Joshua Amimo, Felix Kibegwa, David Harper and Joseph Jung’a

This study genetically characterized five populations of the African catfish (Clarius gariepinus) in Kenya. Samples were obtained from five sites in the country–Athi River hatchery, Kisii Fingerling Production Centre (FPC), Jewlett hatchery, Sagana Hatchery Station and Lake Baringo. DNA was extracted from tissue samples, followed by amplification and sequencing of the dloop region. Haplotype diversities, phylogenetic structure and variation at the dloop region of mitochondrial DNA were assessed.

Mitochondrial DNA analyses indicated that the sampled species showed genetic diversity between its populations. The genetic results were congruent indicating the differences in diversities and haplotype similarities of catfish samples from different sites. The Sagana, Kisii FPC, Jewlett and Baringo population cluster overlapped indicating possibly shared source of brood stock. The Athi river population was in a different cluster and its distinctiveness is attributed to imported brood stock. Both Athi River hatchery and Lake Baringo populations were highly variable and has great potential for production.