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The Effects of NO and AgNO3 on Cell Growth and Salidroside Synthesis in Rhodiola Sachalinensis A.Bor. Cell Suspension Culture
AI Jiang-ning, Zhou Bin and JIA Jing-ming
The purpose of this work was to investigate the effect of abiotic elicitors on the production of salidroside in Rhodiola sachalinensis A.Bor. Different concentration of each elicitor was respectively added into the cell suspension culture in different periods of cell culture. The content of salidroside was determined by high performance liquid chromatography (HPLC). NO could enhance cell growth and the synthesis of salidroside, whereas AgNO3 inhibited cell growth, and promoted the synthesis of salidroside. 50 ?mol/L of SNP as the donator of NO and 60 ?mol/L of AgNO3 were added into cell suspension culture on the 12th day. And the contents of salidroside were significantly increased up to 2.2 fold and 2.0 fold respectively. Therefore the elicitation by NO and AgNO3 can effectively promote accumulation of the secondary metabolite in plant cell culture.