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Wide Hybridization and Embryo-Rescue for Crop Improvement in Capsicum
C.Debbarama,V. K. Khanna, W. Tyagi, M. Rai and NT. Meetei
Wide-hybridization is an important tool in the hands of the plant breeder and a cytogeneticist. It is the first step to transfer genes of the wild species into the cultivated ones. In Meghalaya, chilli is the third most important spice crop after ginger and turmeric. However, diseases namely, tobacco mosaic virus, root rot, tomato spotted wilt virus, etc. lead to a considerable decline in yield. Capsicum chinense, C. annuum and C. frutescens were crossed with each other and embryo rescue was done between 27-33 days after pollination. The highest percentage of embryo growth was observed in MS medium with 0.5 mg/l GA3 and 0.05 mg/l NAA. Hybrid plants were obtained and their hybridity was confirmed using both morphological and RAPD markers.