食品加工与技术杂志

抽象的

Analytical Method Optimization and Determination of Sulfonamides, Chloramphenicol and Tetracyclines Drug Residues in Chicken Meat across Egypt

Mahmoud Abd Elkhabeer, Gouda A. Ramadan Gouda, Lamia Ryad, Eglal R. Souaya

This study was carried out to develop an accurate analytical method for the determination of 17 Sulfonamides (SAs), 4 Tetracyclines (TCs) and Chloramphenicol (CAP) residues to be used in a monitoring program for the analysis of studied veterinary drugs in chicken samples from different poultry farms of Egypt. Instrument linearity was established using a multi-level calibration curve from (1 to 100) μg/L for Sulfonamides and Tetracyclines and from (0.1 to 20) μg/L for Chloramphenicol; the correlation coefficient was ≥ 0.995 for all compounds. Methods linearity has been studied using different concentration levels which lie in between the calibration points. The method proved to be linear for all compounds from Limit of Quantitation (LOQ) to the highest level. The limit of quantitation was 10 μg/L for Sulfonamides and Tetracyclines and 0.2 μg/L for Chloramphenicol. Method accuracy was studied using various Certified Reference Materials (CRMs) and results were found to be valid within the acceptable limits. A total of 60 fresh samples from different poultry farms in Egypt were tested for the presence of studied compounds using liquid Chromatography-Mass Spectrometry (LC-MS/MS). Results showed that there were no positive samples detected except two samples contaminated with doxycycline but lower than the Maximum Residue Limits (MRLs) established by the European database (100 μg/kg). Hence, more monitoring and risk assessment studies required to cover new generations of veterinary drugs.